A1125-01G

Akt1 (Rac PKa, PKBa)

Size

£843.00

SKU:: A1125-01G
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: E. coli-derived recombinant protein corresponding to Met1-Glu149 from human Akt1.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKB-Alpha), Akt2 (PKB-Beta), and Akt3 (PKB-Gamma). Akt1 is the most ubiquitously expressed family member. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.