A1124-54

Akt substrate, 160kD (AS160, KIAA0603, TBC1 domain family member 4, Rab GAP) (Not for Export EU)

Size

£825.00

SKU:: A1124-54
Application:: WB
Purity:: Serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated, synthetic peptide corresponding to aa1178-1189 (C-PTNDKAKAGNKP) of rat Akt substrate of 160kD (AS160) (Rab-GAP) with a N-terminal cysteine for conjugation purposes. Species Sequence Homology: rat and canine.
Format:: Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Extra Details:: AS160 is an 160kD Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 detected AS160 (Rab-GAP) in RIPA lysates from 3T3/L1 cells differentiated into adipocytes by MDI treatment. Also detects AS160 (Rab-GAP) in HEK293 lysates. Optimal dilutions to be determined by the researcher. Positive Control: MDI-treated 3T3/L1 cell lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.