A1124-08A

Akt (PKB, RAC, Related to PKA and PKC)

Size

£570.00

SKU:: A1124-08A
Application:: WB
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: E. coli-expressed mouse Akt alpha (aa 375-479)
Format:: Supplied as a lyophilized powder from 0.09% sodium azide. Reconstitute with 200ul sterile ddH2O.
Extra Details:: Akt (PKB, or RAC (Related to PKA and PKC), is the cellular homologue of the viral oncoprotein v-Akt. Akt is a serine / threonine kinase that consists of an N-terminal PH domain, a kinase domain and a C-terminal domian similar to that of the PKC family. Akt alpha contains 480 amino acids, and can be activated by receptor tyrosine kinases through the PI 3-kinase pathway. Akt has been shown to suppress apoptosis. Recent study suggests that Akt may also be part of the NF-kB activation pathway. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.