A1124-03W
Akt1 (Rac PKa, PKBa)
Size
£767.00
- SKU:
- A1124-03W
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Sf 21-derived recombinant human v-akt murine thymoma viral oncogene homolog 1 aa2-480.
- Clone:
- 9H51
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKBα), Akt2 (PKBβ), and Akt3 (PKBγ). Akt1 is the most ubiquitously expressed family member. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 8-25ug/ml to detect Akt1 in fixed human peripheral blood mononuclear cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice