A1124-02N

Akt, Phosphorylated (Thr473) (Rac PKa, PKBa)

Size

£927.00

SKU:: A1124-02N
Molecular Weight:: 56
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to residues surrounding Thr308 of human AKT1 protein.
Clone:: 18F3.H11
Format:: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Extra Details:: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory- lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: 1 inhibition of apoptosis; 2 promotion of proliferation. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:20,000. Shows minimal reactivity against non-phosphorylated form of the immunizing peptide. Western Blot: 1:500-1:3,000 Immunohistochemistry: 20ug/ml.. Use formalin-fixed, paraffin-embedded tissue sections; no pre-treatment of sample required. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice