AIRE (Autoimmune Regulator, Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein Homolog, APECED Protein Homolog)

Size

£843.00

SKU:: A1059-88G
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: E. coli-derived recombinant protein corresponding to Ser476-Ser552 from mouse Autoimmune Regulator/AIRE. Species sequence homology: human (65%), rat (91%).
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: AIRE (Autoimmune regulator; also APECED) is a 58-60kD transcriptional regulator that exhibits tight regulation of its expression. It is widely expressed in lymphoid organs, but is most pronounced in thymic medullary epithelium, where it induces the expression of organ-specific antigens that are used to sequester and delete autoreactive lymphocytes. Mouse AIRE is 552aa in length. It contains multiple domains, including an HSR domain with two LXXLL motifs (aa1-106), an NLS (aa114-134), one SAND domain (aa182-282) and two PHD zinc-finger regions (aa298-345; 434-475). AIRE will homodimerize and tetramerize. There are multiple potential isoforms that involve various combinations of deletions of aa265-268, 368-426 and Lys296, plus a 42aa substitution for aa368-552. Over aa476-552, mouse AIRE shares 91% and 65% aa identity with rat and human AIRE, respectively. Applications: Suitable for use in Western Blot Other applications not tested. Recommended Dilution: Western Blot:1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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