AIF1 (Allograft Inflammatory Factor 1, AIF-1, Ionized Calcium-binding Adapter Molecule 1, Protein G1, G1, IBA1)

Size

£642.00

SKU:: A1059-86G
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Peptide corresponding to C-NKQFLDDPKYSSDED at the internal region of human AIF1. Species Sequence Homology: canine and rat.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: AIF-1 is a 17kD IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis. Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region. Applications: Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:32,000 Western Blot: 0.5-1.5ug/ml, using human brain (Frontal cortex) and human peripheral blood mononucleocyte lysates (~17kD). Immunohistochemistry (Paraffin): 2-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice