A1059-72Y
Ah Receptor (Aryl Hydrocarbon Receptor, AHR)
Size
£837.00
- SKU:
- A1059-72Y
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to amino acid 12-31, (12)RKRRKP(17)V(22)KPIPAEGIK(31) from mouse AHR protein with amino acids 18-21 omitted.
- Clone:
- 8J216 (RPT9)
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Extra Details:
- The aryl hydrocarbon receptor (AhR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AhR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the Ah Receptor. Studies indicate that in non-ligand activated cells, AhR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AhR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AhR followed by formation of a heterodimer with AhR nuclear translocator protein (Arnt). The AhR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1. Applications: Suitable for use in ELISA, ChIP, Flow Cytometry, Western Blot, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:10-1:500. Staining of rat liver results in strong cytoplasmic and some nuclear staining. Immunoprecipitation: A single ~95kD protein representing AHR from Hepa 1 cytosol. Immunocytochemistry/Immunofluorescence: 1:10-1:100 Western Blot: 1:500-1:2000 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Positive Control: AHR Overexpression Lysate Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice