AGR2 (Anterior Gradient Protein 2 Homolog, AG-2, hAG-2, HPC8, Secreted Cement Gland Protein XAG-2 homolog, AG2, UNQ515/PRO1030) (Azide-Free)

Size

£639.00

SKU:: A1059-59F2
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptides corresponding to aa55-72 (TQTYEEALYKSKTSNKPL) of human AGR2 (NP_006399.1). Species sequence homology: bovine, chicken, chimpanzee, canine, orangutan, rat, xenopus.
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kD to ~600kD. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:100 Immunohistochemistry (formalin fixed paraffin embedded): 1:100 Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice