A0909-39P
Adrenomedullin (ADML)
Size
£761.00
- SKU:
- A0909-39P
- Application:
- IHC-Fr
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ala22-Gly147 from human Adrenomedullin, expressed in E. coli.
- Clone:
- 11n3
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Adrenomedullin (ADM) is a secreted, monomeric, 6kD member of the adrenomedullin family of molecules. It is widely expressed, being found in smooth muscle cells, endothelium, adrenal medulla chromaffin cells, fibroblasts and keratinocytes. Adrenomedullin has multiple functions including vasodilation, the maintenance of vascular integrity, and the suppression of inflammatory mediator secretion. The human Adrenomedullin preproprecursor is 185aa in length. It contains a 21aa signal sequence, a processed 20aa peptide termed PAMP aa22-41, an N-terminal propeptide aa45-92, the Adrenomedullin precursor (amidation is required for maturation) aa95-147, and a C-terminal propeptide aa148-185. The Adrenomedullin precursor with a terminal Gly147 circulates naturally with bioactive, mature amidated Adrenomedullin aa95-146. Depending upon the tissue, truncated forms of Adrenomedullin likely also occur, including variants spanning aa120-146,128-146. Over aa22-147, the human Adrenomedullin proprecursor shares 70aa identity with mouse Adrenomedullin proprecursor. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8-25ug/ml using Immersion fixed paraffin-embedded sections of human heart. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice