A0901-25E

ADPN (Adiponutrin, Patatin-like Phospholipase Domain Containing 3, Acylglycerol O-acyltransferase, C22orf20, Calcium-independent Phospholipase A2-epsilon, dJ796I17.1, FLJ22012, iPLA(2)epsilon, iPLA2-epsilon)

Size

£1,093.00

SKU:: A0901-25E
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to aa436-481 of human Adiponutrin.
Format:: Supplied as a lyophilized powder from PBS. Reconstitute with 200ul of sterile PBS.
Extra Details:: Adiponutrin is one of three recently identified adipocyte lipases. Surprisingly, these proteins also retain transacylase activity, a hitherto unknown pathway of triacylglycerol synthesis in the adipocytes. This may enable them to participate in both anabolic and catabolic processes. The adiponutrin gene (ADPN) is downregulated by fasting and upregulated by refeeding, suggesting a role in lipogenesis. Experiments in human adipocytes confirmed that the gene is upregulated in response to insulin in a glucose-dependent fashion. Obese subjects had increased levels of subcutaneous and visceral abdominal adipose tissue ADPN mRNA. Visceral ADPN mRNA expression was correlated to measures of insulin sensitivity (fasting insulin and homeostasis model assessment). Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:6400, 0.156ug/ml Optimal dilutions to be determined by the researcher. AA Sequence: Cys-Pro-Ala-Glu-Thr-Lys-Ala-Glu-Ala-Thr-Pro-Arg-Ser-Ile-Leu-Arg-Ser-Ser-Leu-Asn-Phe-Phe-Leu-Gly-Asn-Lys-Val-Pro-Ala-Gly-Ala-Glu-Gly-Leu-Ser-Thr-Phe-Pro-Ser-Phe-Ser-Leu-Glu-Lys-Ser-Leu Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice