A0901-25A
ADPN (Adiponutrin, Patatin-like Phospholipase Domain containing 3)
Size
£889.00
- SKU:
- A0901-25A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- E. coli-derived recombinant protein corresponding to Arg160-Arg349 of human Adiponutrin/PNPLA3.
- Format:
- Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 500ul PBS.
- Extra Details:
- Human ADPN (Adiponutrin; also PNPLA3 and patatin-like phospholipase domain-containing protein 3) is a 53kD member of the PNPLA family of phospholipase A2 enzymes. It is expressed in white adipocytes. Adiponutrin is elevated in response to insulin and appears to act not as a phospholipase, but a molecule involved in anabolic pathways. ADPN is likely to be a type II transmembrane protein that is 481aa in length. It contains one patatin domain (aa10-179) that is associated with in vitro enzyme activity and a transmembrane segment between aa42-62. There appear to be two splice variants. One shows a four aa deletion of aa59-62 and a second shows an alternate start site at Met379. Over aa 160-349, human ADPN shares 61% aa identity with mouse Adiponutrin. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Immunohistochemistry: 10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice