A0859-90B
Adaptin, alpha (AP-2 Complex Subunit alpha-1, 100kD Coated Vesicle Protein A, Adapter-related Protein Complex 2 alpha-1 Subunit, Adaptor Protein Complex AP-2 Subunit alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin Assembly Protein Complex 2 alpha-A Large Chain, Plasma Membrane Adaptor HA2/AP2 Adaptin alpha A Subunit, AP2A1, ADTAA, CLAPA1)
Size
£512.00
- SKU:
- A0859-90B
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- AP-2 adaptor polypeptides from bovine brain.
- Clone:
- 10K99
- Format:
- Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.
- Extra Details:
- Alpha Adaptin, also known as adaptor related protein complex, alpha 1 subunit. The alpha adaptins are exclusively found in the endocytic coated vesicles. Huntingtin-interacting protein 1 (HIP1) is enriched in membrane-containing cell fractions and has been implicated in vesicle trafficking. Waelter et al. (2001) identified 3 HIP1-associated proteins, clathrin heavy chain (CLTC) and alpha-adaptin A and C. Coat proteins of approximately 100kD (adaptins) are components of the adaptor complexes which link clathrin to receptors in coated vesicles. The alpha-adaptins separate into two bands on SDS gels. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1.2% sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice