A0859-61E5

ADAMTS5 (Aggrecanase 2, A Disintegrin And Metalloproteinase with Thrombospondin-3 motif)

Size

£832.00

SKU:: A0859-61E5
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Ser262-Pro622 of human ADAMTS5, expressed in NSO cells.
Clone:: 9H41
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: ADAMTS5, also known as aggrecanase-2 and ADADMTS11, ADAMTS5 precursor consists of multiple domains. The purified rhADAMTS5 starts at the N-terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of rhADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml under non-reducing conditions Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human ADAMTS5 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.