A0859-41D

ADAM 23 (A Disintegrin And Metalloproteinase 23)

Size

£812.00

SKU:: A0859-41D
Application:: IHC-Fr
Purity:: Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Ser60-His585 from human ADAM23, expressed in CHO cells.
Clone:: 9H37
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul PBS.
Extra Details:: ADAM23 (a disintegrin and metalloprotease domain 23; also MDC3) is a 100kD member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum, and serves as a counter-receptor for alpha v beta 3 integrin. The human ADAM23 proprecursor is a 773aa type I transmembrane (TM) protein. It contains a 227aa cleavable proregion (aa60-286) and a 506aa extracellular domain (ECD) (aa287-792) that is part of a 70kD mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa299-494), an integrin-binding disintegrin region (aa511-585), and a Cys-rich domain (aa589-611). Two splice variants exist. One shows an in-frame 46aa substitution for aa787-832 that generates a soluble form, while a second shows an in-frame 31aaTM substitution for aa787-817. Over aa1-585, human ADAM23 is 92% aa identical to mouse ADAM23. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human spleen Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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