A0859-25Q1
ADAM12 (MCMP, MLTN, MLTNA, MCMPMltna, A disintegrin and metalloproteinase domain 12, Meltrin alpha)
Size
£825.00
- SKU:
- A0859-25Q1
- Application:
- IP
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Arg29-Ser513 from human ADAM12 expressed in CHO cells. Swiss/UniProt Accession: O43184.
- Clone:
- 11n1
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- ADAM12 is a 120kD type I transmembrane glycoprotein member of the metzincin family of proteases. It is synthesized as a 909aa preproprecursor that contains a 25kD proregion aa29-207 and a 92kD mature molecule. Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression. A 68kD soluble splice form exists in pregnancy where 36aa replace the C-terminal 205 aa705-909. This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator. Over aa29-513, human ADAM12 shares 78-79aa identity with bovine and mouse ADAM12, respectively. Applications: Suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 25ug/ml detects ADAM12 in conditioned cell culture medium spiked with recombinant human ADAM12. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice