A0859-25K
ADAM 12 (MCMP, MLTN, MLTNA, MCMPMltna, Meltrin alpha, A disintegrin and metalloproteinase domain 12)
Size
£863.00
- SKU:
- A0859-25K
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to aa29-513 with a Glu36Gln substitution human ADAM12 expressed CHO cell.
- Format:
- Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, pH 7.4, with 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- ADAM12 (A disintegrin and metalloprotease domain 12; also meltrin-α) is a 120kD type I transmembrane glycoprotein member of the metzincin family of proteases. It is synthesized as a 909 amino acid (aa) preproprecursor that contains a 25kD proregion (aa 29-207) and a 92kD mature molecule. Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression. A 68kD soluble splice form exists in pregnancy where 36 aa replace the C-terminal 205 aa (705-909). This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator. Over aa 29-513, human ADAM12 shares 78% and 79% aa identity with bovine and mouse ADAM12, respectively. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunoprecipitation: 25ug/ml Western Blot: 0.1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice