Activin Receptor Type IIA (RIIA)

Size

£934.00

SKU:: A0856-05C
Purity:: Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Ser25-Asp82 from human Activin RIIA, expressed in Sf 21 cells.
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated. Applications: Suitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Neutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml recombinant human Activin A to immobilized recombinant human Act RIIA/Fc Chimera (100ul of a 0.5ng/ml solution was coated in each well) in a functional ELISA assay. Western Blot: 0.1ug/ml Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human prostate cancer tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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