A0855-89
Activin A Precursor
Size
£1,017.00
- SKU:
- A0855-89
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- DNA construct encoding human Activin A pro region fused to mature human Activin beta B chain
- Clone:
- 9H16
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Activins are dimers consisting of two beta subunits. Several types of beta subunits have been described. The same beta subunits also occur in Inhibins as part of a heterodimer with an alpha subunit. The beta subunits are translated as a precursor form that includes an N-terminal pro region which is proteolytically cleaved to yield the mature C-terminal beta subunit. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Activin A precursor is ~15ng/well. Western Blot: 1- 2ug/ml. The detection limit for recombinant human Activin A precursor is ~5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma with B cells obtained from a mouse immunized with a DNA construct encoding the human Activin A pro region fused to mature human Activin beta B chain. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice