Activating Transcription Factor 4 (ATF4, Tax-responsive Enhancer Element B67, Cyclic AMP-dependent Transcription Factor ATF-4, cAMP-dependent Transcription Factor ATF-4, Cyclic AMP-responsive Element-binding Protein 2, CREB-2, cAMP-responsive Element-binding Protein 2, DNA-binding Protein TAXREB67, CREB2)

Size

£642.00

SKU:: A0855-69R
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-EEVRKARGKKRVP, from human ATF4, at C-terminal (NP_001666.2, NP_877962.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Western Blot: 1-3ug/ml, Raji cell lysate observed on ~40kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice