A0761
Actinin, alpha 1 (Non-muscle)
Size
£877.00
- SKU:
- A0761
- Purity:
- Purified by Protein A affinity chromatography from culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human alpha-Actinin
- Clone:
- 0.T.02
- Format:
- Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
- Extra Details:
- The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml. Detects alpha-Actinin in RIPA lysates from human A431 cells. Immunocytochemistry/Immunohistochemistry: 15ug/ml. Reported positive immunostaining for alpha-Actinin in MRC-5 cells fixed with methanol and also in frozen sections. Optimal dilutions to be determined by the researcher. Positive Antigen Control (Included): A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt x Mo I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt x Mo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice