A0761-ML650
Actinin, alpha 1 (Non-muscle) (MaxLight 650)
Size
£1,133.00
- SKU:
- A0761-ML650
- Purity:
- Purified by Protein A affinity chromatography from culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human alpha-Actinin
- Clone:
- 0.T.02
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml. Detects alpha-Actinin in RIPA lysates from human A431 cells. Immunocytochemistry/Immunohistochemistry: 15ug/ml. Reported positive immunostaining for alpha-Actinin in MRC-5 cells fixed with methanol and also in frozen sections. Optimal dilutions to be determined by the researcher. Positive Antigen Control (Included): A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt x Mo I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt x Mo Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice