A0760-89C

ARPC5, Recombinant, Human, aa1-151, His-Tag (Actin-related Protein 2/3 Complex Subunit 5 Protein, Arp2/3 Complex 16kD Subunit 2, ARC16-2)

Size

£512.00

SKU:: A0760-89C
Molecular Weight:: 18.4
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 1mM DTT, 0.1M sodium chloride, 50% glycerol.
Extra Details:: p16-ARC, also known as ARPC5, is a 151 amino acid subunit of the Arp2/3 complex. Thought to play a role in maintaining the integrity of Arp2/3, ARPC5 is a substrate for MAPKAPK-2 which, through phosphorylation of ARPC5, may participate in Arp2/3 regulatory functions and remodeling of the Actin cytoskeleton. Two isoforms of ARPC5 exist due to alternative splicing events. Purified by using conventional chromatography techniques. Source: Recombinant correpsonding to aa1-151 of human ARPC5, fused to His-tag at N-terminus, expressed in E.coli. AA Sequences: MGSSHHHHHH SSGLVPRGSH MSKNTVSSAR FRKVDVDEYD ENKFVDEEDG GDGQAGPDEG EVDSCLRQGN MTAALQAALK NPPINTKSQA VKDRAGSIVL KVLISFKAND IEKAVQSLDK NGVDLLMKYI YKGFESPSDN SSAMLLQWHE KALAAGGVGS IVRVLTARKT V Molecular Weight: ~18.4kD (171aa), confirmed by MALDI-TOF Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.