Actin, gamma (Not for Export EU)

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£806.00

SKU:: A0760-45
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Synthetic peptide corresponding EEEIAALVIDNGSGGSG at the N-terminus of gamma actin, conjugated to KLH. Cellular Localization: Cytoplasm and cytoskeleton
Format:: Supplied as a liquid.
Extra Details:: Actin is a 43kD highly conserved structural protein. There are three main actin isotypes (alpha,beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. There are at least six actin isoforms in vertebrates: cardiac alpha-actin and skeletal-alpha actin (expressed in striated muscle), vascular alpha-actin and enteric gamma-actin (expressed in smooth muscle), and cytoplasmic beta- and gamma-actins. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000. Non-specific bands can occur when A0760-45 is overloaded. Blocking with purified milk proteins preferred over commerical concentrated milk solutions. Detects a 42-43kD band Immunocytochemistry: 1:500-1:2000. Cultured cells fixed with PFA, then permeabilized with methanol. Immunohistochemistry: 1:500-1:2000. Tissues frozen and then fixed with PFA followed by Triton permeabilisation. 10% buffered formalin fixed tissues have also been used Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice