A0760-43B
Actin, beta (HRP)
Size
£672.00
- SKU:
- A0760-43B
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to 15aa from human beta Actin (non-muscle) conjugated to KLH. Epitope: ~N-terminal. Species Sequence Homology: mouse, rat, chicken and frog
- Clone:
- 9j104
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% merthiolate. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 100ul sterile PBS.
- Extra Details:
- Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle, one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:5000-1:10,000 Western Blot: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice