A0760-43A
Actin, beta (HRP)
Size
£486.00
- SKU:
- A0760-43A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to DDDIAALVVDNGSG from beta Actin conjugated to KLH
- Clone:
- 8A103 (2D1D10)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.01% thimerosal. Labeled with Horseradish Peroxidase (HRP). Reconstitute with sterile ddH2O to 0.5mg/ml
- Extra Details:
- Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0-AG14 mouse myeloma cells with spleen cells. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice