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A0760-41A

Actin, beta (Not for Export EU)

Size

£704.00

SKU:
A0760-41A
Purity:
Serum.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Purified (>95%) human platelet (nonmuscle) beta-actin protein.
Format:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute in 100ul PBS.
Extra Details:
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one bactin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18-aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1: 50,00 Control peptide can be used to coat ELISA plates at 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice