A0760-40

Actin, beta

Size

£704.00

SKU:: A0760-40
Purity:: Purified from hybridoma cell culture
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to Ac-Asp-Asp-Asp-Ile-Ala-Ala- Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys from beta Actin at N-terminal, conjugated to KLH. Molecular Weight: 42kD.
Clone:: 2A2.1
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: Beta-actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Because beta-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western Blot. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-1ug/ml. Useful as a loading control for a wide variety of cell extracts including human foreskin fibroblasts or chicken fibroblasts. Immunohistochemistry (Paraffin and frozen): The epitope recognized by the antibody is resistant to paraffin or formalin-fixed tissue sections. Acetone fixation or B5, Methacarn, ethanol or Bouin’s solution may also be used as fixatives. Immunofluorescence (IC): 10-40ug/ml on methanol/acetone fixed samples. Staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membranes associated dense-plaque. Optimal dilutions to be determined by the researcher. Positive Control: Cultured human or chicken fibroblasts Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice