A0760-27B

Actin, alpha, Smooth Muscle

Size

£621.00

SKU:: A0760-27B
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic N-terminus decapeptide of alpha-smooth-muscle isoform of actin.
Clone:: 5F160 (ASM-1)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide.
Extra Details:: Represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation, cultured stress fibers of smooth muscle-derived cells, and some smooth muscle subtype fibroblasts. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry (frozen and paraffin-embedded tissue as well as cytological material). Other applications not tested. Recommended Dilutions: Immunohistochemistry: Ready-to-use on frozen sections. Paraffin embedded sections require protease pretreatment prior to antibody application. Incubate 1 hour at RT; extended with paraffin. Optimal dilution to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.