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A0760-26

Actin, alpha, Smooth Muscle

Size

£754.00

SKU:
A0760-26
Purity:
Purified by Protein A affinity chromatography from ascites.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
N-terminal decapeptide of the alpha smooth muscle isoform of Actin; acetylated at the N-terminus. Epitope: Acetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth Actin. Cellular Localization: Cytoplasmic
Clone:
0.N.5 (1A4)
Format:
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Extra Details:
Actin is a highly conserved skeletal protein which is in both muscle and nonmuscle cells. The mammalian tissues express at least 6 unique actin isotypes. Alpha isotype is found exclusively in muscle cells including alpha-skeletal, alpha-cardiac, and alpha and gamma smooth muscle actin. Beta and gamma nonmuscular actins have been observed in non-muscle cells. Applications: Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): 1:800 for 20 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. Optimal dilutions to be determined by the researcher. Positive Control: Smooth muscle or leiomyosarcoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice