A0759-25
Actin, Cardiac, Fetal
Size
£800.00
- SKU:
- A0759-25
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic N-terminus decapeptide of cardiac isoform of actin.
- Clone:
- 5F152
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
- Extra Details:
- Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbilical cord. Applications: Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:500 (see Note below). Western Blot: 1:1000 (using ECL) Note: It is necessary to include 1M sodium chloride for application in Western Blot and ELISA to enhance specificity. Immunohistochemistry (Frozen, Paraffin): 1:10. It is necessary to include 0.5M sodium chloride in all washing buffers for application on frozen tissue. This is not necessary for paraffin sections. Microwave treatment is necessary for paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O/PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice