A0759-10H
Actin, pan
Size
£927.00
- SKU:
- A0759-10H
- Purity:
- Ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified chicken actin protein.
- Clone:
- 11C73
- Format:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Extra Details:
- Muscle (from Latin musculus "little mouse") is contractile tissue of the body and is derived from the mesodermal layer of embryonic germ cells. There are three types of muscles (skeletal, cardiac and smooth): Skeletal muscle or "voluntary muscle" is anchored by tendons to bone. Smooth muscle or "involuntary muscle" is found within the walls of organs and structures such as the esophagus, stomach, intestines, bronchi, uterus, urethra, bladder, and blood vessels, and unlike skeletal muscle, smooth muscle is not under conscious control. Cardiac muscle is also an "involuntary muscle" but is a specialized kind of muscle found only within the heart. Cardiac and skeletal muscle are "striated" in that they contain sarcomeres and are packed into highly-regular arrangements of bundles; smooth muscle has neither. Muscle is mainly composed of muscle cells. Within the cells are myofibrils; myofibrils contain sarcomeres, which are composed of actin and myosin. All three muscles use the movement of actin against myosin to create contraction. Smooth muscle cells are generally arranged in sheets or bundles and connected by gap junctions. In order to contract the cells contain intracellular contractile filamentous proteins called actin and myosin. Smooth muscle does not contain the proteins troponin or titin, and caldesmon and calponin are significant proteins expressed within smooth muscle. Actin filaments attach to the sarcolemma by focal adhesions or attachment plaques and attach to other actin filaments via dense bodies (acting much like Z-lines in striated muscle). Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three alpha-actins: alpha-skeletal, alpha-cardiac, and alpha-smooth muscle), one beta-actin (beta-non-muscle), and two gamma-actins (gamma-smooth muscle and gamma-non-muscle). Actin isoform are >90% conserved, except in the N-terminal 18aa (50-60% homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots. Applications: Suitable for use in ELISA, Immunohistochemistry, and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-50,000; 50-200ng control antigen/well Western Blot: 1:1500-1:2,000 (chemiluminescence) Immunohistochemistry: 1:2,000-1:5,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice