A0759-10F
Actin, pan (alpha, beta, gamma) (Cy3)
Size
£704.00
- SKU:
- A0759-10F
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to human 11-aa-MAPS (SGPSIVHRKCF) of actin.
- Clone:
- 11C68
- Format:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Extra Details:
- Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are ~90% conserved, except in the N-terminal 18aa (50-60%homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots. Applications: Suitable for use in Immunofluorescence, FLISA and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:50-1:200 FLISA: 1:10,000-1:50,000; using 50-200g control antigen/well Western Blot: 1500-1:2,000 (Chemiluminescence) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice