A0759-07-APC

Actin (APC)

Size

£1,088.00

SKU:: A0759-07-APC
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified chicken gizzard actin. Epitope: Appears to be located in the N-terminal two thirds of the actin molecule, possibly near aa50-70.
Clone:: 2Q1055 (C4)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Actin is an abundant cytoskeletal protein found in all cells. The protein’s 42kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction. In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis and phagocytosis. Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific. Applications: Suitable for use in Western Blot and Immunocytochemistry/Immunofluorescence. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:5000; HEK293 cell lysates were probed with A0759-07 and visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunocytochemistry (IF): 1:500; HeLa and NIH/3T3 cells were probed with A0759-07 and DAPI for nuclear staining. Visualized using an IgG (Cy3) donkey anti-mouse. Positive staining pattern reflects both membrane and cytoplasmic staining. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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