A0650-01L

ACK1, N-terminal GST-Tag, Recombinant, Human (TNK2, Tyrosine Kinase, non-receptor 2, Tyrosine Kinase non-receptor protein 2, ACK, ACK1, Activated CDC42 kinase 1, FLJ44758, FLJ45547, p21cdc42Hs)

Size

£912.00

SKU:: A0650-01L
Molecular Weight:: 68
Purity:: Purified ≥70%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 3mM DTT, 20% glycerol.
Extra Details:: Source: Recombinant corresponding to full length human rAck1 with N-terminal GTS tag expressed in Sf9 insect cell systems (NM_005781). Specific Activity: 88pmol/min/mg Assay conditions: Add 50ul reaction mix (ACK1 diluted in 1x kinase buffer supplemented with 0.2mM ATP) to wells coated with substrate. Incubate for 1hour. Add Europeum-labeled anti p-Tyr antibody and incubate for 1hour. Time resolved fluorescence measured at RT at lambda-exc=330±40nm and lambda-em=620±10nm. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.