A0560-19C
ACP1 (Low Molecular Weight Phosphotyrosine Protein Phosphatase, LMW-PTP, LMW-PTPase, Adipocyte Acid Phosphatase, Low Molecular Weight Cytosolic Acid Phosphatase, Red Cell Acid Phosphatase 1)
Size
£825.00
- SKU:
- A0560-19C
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- E.coli-derived recombinant protein corresponding to human ACP1, Ala2-His158.
- Clone:
- 11C1
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Low molecular weight protein tyrosine phosphatase (LMW-PTP), also known as Acid Phosphatase 1 (ACP1), is an 18kD cytosolic phosphatase that is unrelated to other tyrosine phosphatases, such as PTP1B. Tyrosine phosphorylation of LMW-PTP increases its activity 20-fold and affects its ability to dephosphorylate targets such as the PDGF receptor. Cancer cells overexpressing LMW-PTP have a higher proliferative rate and generate tumors that are larger than untransfected controls. Colon adenocarcinomas induced by dimethylhydrazine also have elevated levels of LMW-PTP, suggesting that this phosphatase may be a marker for oncogenic transformation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice