A0528-19G2

B3GNT2 (UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2, BGnT-2, Beta-1,3-Gn-T2, Beta-1,3-N-acetylglucosaminyltransferase 2, Beta3Gn-T2, Beta-1,3-N-acetylglucosaminyltransferase 1, BGnT-1, Beta-1,3-Gn-T1, Beta3Gn-T1, Beta-1,3-galactosyltransferase 7, Beta-1,3-GalTase 7, Beta3Gal-T7, Beta3GalT7, b3Gal-T7, Beta-3-Gx-T7, UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 7, UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 7, B3GALT7, B3GNT1)

Size

£837.00

SKU:: A0528-19G2
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant corresponding to aa29-397 from human B3GNT2 expressed in NSO cell line.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: B3-GNT (also B3GNT-2) is an 80kD (45kD without glycosylation) type II transmembrane Golgi enzyme that belongs to the glycosyltransferase 31 family of enzymes. It catalyzes the transfer of N-acetyl glucosamine from UDP-GlcNAc onto Gal beta 3(GlcNAc beta 6)GalNAc-mucin. It functions in the elongation and branching of O-linked oligosaccharide chains of mucin glycoproteins. Over aa29-397, human B3GNT2 shares 88% aa sequence identity with rat B3GNT2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.