A0528-19E
N-Acetylglucosamine, O-linked
Size
£626.00
- SKU:
- A0528-19E
- Purity:
- Ascites
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Heat killed, pepsin treated group A streptococci (Streptococcus pyrogenes)
- Clone:
- 8J157 (HGAC85)
- Format:
- Supplied as a liquid, 0.09% sodium azide.
- Extra Details:
- O-GlcNAc (O-linked N-acetylglucosamine) is one of the most abundant posttranslational modifications on nuclear and cytoplasmic proteins. Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-GlcNAc residues attached to serine or threonine residues. This modification occurs via the O-GlcNAc transferase (OGT). There appears to be a competitive modification at these residues. Either the residues are glycosylated or they are phosphorylated. Because of the significance of phosphorylation in cancer research, such competition can be of major interest. O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may also obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling. Recent research has revealed that the O-GlcNAcylation of the EGF repeats of Notch are modified via a novel O-GlcNAc transferase, EOGT1. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Chromatin Immunoprecipitation: 1:120-1:500 ELISA: 1:100-1:200 Immunofluorescence (IC): 1:100-1:200 Immunohistochemistry (paraffin): 1:200 Immunoprecipitation: 1:10-1:500 Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice