A0528-08

N-Acetylgalactosamine 4 Sulfate 6-O-Sulfotransferase (GalNAc4S-6ST)

Size

£812.00

SKU:: A0528-08
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant..
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Mouse myeloma cell line NS0-derived recombinant protein corresponding to Ser99-Thr561 from human sulfotransferase 15/CHST15.
Clone:: 8H167
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: N-acetylgalactosamine 4 sulfate 6-O-sulfotransferase (GalNAc4S-6ST) also known as B cell RAG-associated protein (BRAG), is a type II transmembrane Golgi protein that catalyzes the transfer of sulfate onto GalNAc (4 SO4) in chondroitin sulfate A and dermatan sulfate. The 469 aa rhGalNAc4S-6ST predicts a molecular mass of 55kD and migrates as two bands with molecular masses of approximately 68 and 73kD. The amino acid sequence of human GalNAc4S-6ST is 99%, 93%, 88% and 79% identical to that of chimpanzee, rat/mouse, canine, and chicken. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhGaINAc4S-6ST is ~ 10ng/well. Western Blot: 1ug/ml detection limit for rhGaINAc4S-6ST is ~ 5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice