A0225-05A
AcCoA Carboxylase (Ser79), phosphorylated
Size
£856.00
- SKU:
- A0225-05A
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide containing (…SSM[pS]GL), in which [pS] corresponds to phospho-Serine at residue 79 of rat AcCoA Carboxylase conjugated to KLH.
- Clone:
- 3J1 (BK102)
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. Also available without sodium azide. See A0225-05A1.
- Extra Details:
- Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. The protein is localized to the cytoplasm in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml. Detects phospho-Acetyl CoA Carboxylase (Ser79) in mouse heart cytosolic lysates. Mouse heart cytosolic lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0225-05A. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Recommended Positive Control: Acid Extracts from HeLa cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice