A0225-05A-BIOTIN
AcCoA Carboxylase (Ser79), phosphorylated (Biotin)
Size
£1,138.00
- SKU:
- A0225-05A-BIOTIN
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide containing (…SSM[pS]GL), in which [pS] corresponds to phospho-Serine at residue 79 of rat AcCoA Carboxylase conjugated to KLH.
- Clone:
- 3J1 (BK102)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. The protein is localized to the cytoplasm in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml. Detects phospho-Acetyl CoA Carboxylase (Ser79) in mouse heart cytosolic lysates. Mouse heart cytosolic lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0225-05A. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Recommended Positive Control: Acid Extracts from HeLa cells Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice