A0004-75A-HRP

ABIN-1 (A-20 binding Inhibitor of Nuclear Factor-kappaB (NF-kB) Activation) (HRP)

Size

£1,119.00

SKU:: A0004-75A-HRP
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to the N-terminal region of the human, mouse and rat ABIN-1 protein.
Clone:: 6A2
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
Extra Details:: A20-binding inhibitor of NFkB activation (ABIN-1) is involved in NFkB-dependent gene expression and plays an important role in numerous cellular processes including stress responses, inflammation and cell proliferation. ABIN-1 increases cell surface CD4 (T4) antigen expression, and it interacts with HIV-1 matrix protein and is packaged into virions. Overexpression of ABIN-1can .inhibit viral replication. ABIN-1 may also regulate matrix nuclear localization, both nuclear import of PIC (Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. ABIN-1 is strongly expressed in peripheral blood lymphocytes, spleen and skeletal muscle, and is weakly expressed in the brain. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA (Immunogen): 0.1-1ug/ml Immunoprecipitatio (Human): 7-10ug/test Western Blot (Human): 1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Control: HEK-293 cell lysate. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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