ABCG1 (ATP-binding Cassette Sub-family G Member 1, ATP-binding Cassette Transporter 8, ABC Transporter 8, ABC8, Homolog of Drosophila White, MGC34313, White Protein Homolog, WHITE1, Wht1) (HRP)

Size

£766.00

SKU:: A0004-11H
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to an internal region of human ABCG1 (between residues 300-400).
Format:: Supplied as a liquid in PBS. No preservative added. Labeled with horseradish peroxidase (HRP).
Extra Details:: ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. The ABC transporter ABCG1 (ABC8) is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages and is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. In contrast, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1. Applications: Suitable for use in Western Blot and Immunohistochemistry. Not recommended for Immunoprecipitation.. Other applications not tested. Recommended Dilution: Western Blot: Mouse peritoneal macrophages treated with 22-R-hydroxycholesterol and T0901317 (stimulating ABCG1 expression). Recognizes a band at ~75.6 kD Optimal dilution determined by the researcher. Storage and Stability: Store product at 4°C, do not freeze. Stable for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice