ABCE1 (ATP-binding Cassette Sub-family E Member 1, 2'-5'-oligoadenylate-binding Protein, HuHP68, RNase L Inhibitor, Ribonuclease 4 Inhibitor, RNS4I, RLI, RNASEL1, RNASELI, RNS4I, OK/SW-cl.40)

Size

£642.00

SKU:: A0004-03F
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-KLNSIKDVEQKK, from human ABCE1, at C-terminal (NP_002931.2, NP_001035809.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Antagonizes the binding of 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) by RNase L through direct interaction with RNase L and therefore inhibits its endoribonuclease activity. May play a central role in the regulation of mRNA turnover. Antagonizes the anti-viral effect of the interferon-regulated 2-5A/RNase L pathway. May act as a chaperone for post-translational events during HIV-1 capsid assembly. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:8000 Western Blot: 0.3-1ug/ml, observed in lysates of cell lines A431 on ~70kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.