A0000-33C
A33 (GPA33, Glycoprotein A33 Transmembrane Cell surface A33 antigen precursor, MGC129986, MGC129987) (Biotin)
Size
£876.00
- SKU:
- A0000-33C
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant corresponding to lle22-Val235 of human A33 extracellular domain, expressed in NS0 cells.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 50ug BSA. Reconstitute with 250ul sterile PBS.
- Extra Details:
- Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.25ug/10e6 cells using human HT-29 human colon carcinoma cell line. Western Blot: 0.1ug/ml using recombinant human A3 Optimal dilutions to be determined by the researche Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice