A0000-33
A33 (GPA33, Cell Surface A33 Antigen)
Size
£856.00
- SKU:
- A0000-33
- Purity:
- Purified by immunoaffinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Ile22-Val235 from human A33 expressed in NS0 cells.
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. Applications: Suitable for use in Flow Cytometry, Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10e6 cells. Tested using TH-29 human colon carcinoma cell line Western Blot: 0.1ug/ml using recombinat human A33 Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human colon Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice