Interleukin 4 Receptor alpha (IL-4R alpha, CD124)

Size

£863.00

SKU:: 592484
Purity:: Purified by Protein A affinity chromatography from ascites. Endotoxin: ≤0.1EU/ug (LAL).
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Gly24-His232 from human interleukin 4 receptor, expressed in Sf21 cell line
Clone:: 24D2
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Interleukin 4 receptor (IL-4 R) is the transmembrane ligand binding subunit of the heterodimeric IL-4 receptor complex. In the type I receptor complex, IL-4 R associates with the common gamma chain, whereas in the type II receptor complex, it associates with IL-13 R alpha 1. IL-4 R is expressed by B cells, T cells, monocytes and macrophages. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry and Neutralization. Other applications not tested. Recommended Dilutions: Neutralization: Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. (1). The ND50 is typically 3-6ng/mL in the presence of 0.2ng/mL recombinant human IL‑4. Flow Cytometry: 0.25ug/10e6 cells Immunohistochemistry: 8-25ug/ml for immersion fixed paraffin-embedded sections of human lymph node Immunocytochemistry: 5-15ug/ml for immersion fixed HDLM-2 human Hodgkin's lymphoma cell line Western Blot: 1ug/ml detects human IL-4R under non-reducing conditions Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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