592473-BIOTIN

Claudin 2 (CLDN2, SP82) (Biotin)

Size

£1,023.00

SKU:: 592473-BIOTIN
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Full-length recombinant protein corresponding to human Claudin-2. Epitope: Extracellular domain
Clone:: 1A2
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: Claudin-2 (UniProt: P57739; also known as SP82) is encoded by the CLDN2 (also known as PSEC0059, SP82, UNQ705/PRO1356) gene (Gene ID: 9075) in human. Claudin-2 is a multi-pass membrane protein with three cytoplasmic domains, two extracellular domains, and four transmembrane domains. It can exist as homopolymer or as a heteropolymer with other claudins mediated through the first extracellular domain (aa29-81). These interactions are achieved largely through electrostatic interactions with Asp65 and with somewhat weaker interaction with electrons of the Tyr67. Claudin-2 is expression has been reported in liver, kidney, and small intestine. It is a component of epithelial tight junctions (TJ) that plays a key role in TJ-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Its expression is shown to be enhanced in subjects with inflammatory bowel disease. Treatment with TNF-a is also reported to upregulate the expression of Claudin-2 via PI3-kinase signaling that reduces transepithelial electrical resistance (TEER). 592473 is shown to reduce TNF-a-induced reduction in TEER and restore TJ integrity. (Ref.: Takigawa, M., et al. (2017). J. Pharmacol. Exp. Ther. 363(3); 444-451). Applications: Suitable for use in Flow Cytometry and Inhibition Assays. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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