CD40

Size

£840.00

SKU:: 592462
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant mouse CD40 fusion protein.
Clone:: FGK45 (recomb)
Format:: Supplied as a liquid in PBS, 0.02% Proclin 300.
Extra Details:: Applications: Suitable for use in Flow Cytometry, Western Blot, Activating, Agonist and Functional Assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Application Notes: FGK45 is an agonistic antibody that has been shown to activate CD40 expressing antigen presenting cells (APCs) what might promote anti-tumor T cell responses. For instance, the rat IgG2a version of this antibody (592462) was used to promote maturation of APCs what improved the ability of the macrophages to fight a tumor (Luheshi et al., 2016; pmid: 26918344). Another group used the same antibody to stimulate T-cells in a study on a tumor vaccine (Bartkowiak et al., 2015; pmid: 26351680). Others used ths clone as well to induce potent anti-tumor activity (Carmi et al., 2015; pmid: 25924063). On the other hand, it can also be used to inhibit CD40/CD154 interaction in vitro and in vivo. Furthermore, this clone can be also utilized to identify CD40-positive cells by flow cytometry (Rolink et al., 1996; pmid: 8885865). Administering this antibody can be also utilized to induce inflamatory bowel disease-like characteristics and create a mouse IBD model to study this class of diseases (Bauche et al., 2018; pmid: 30097293). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice