Troponin I, aa27-40

Size

£512.00

SKU:: 591634
Purity:: Purified by immunoaffinity chromatography. ≥95% (HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Purified Human Cardiac Troponin, I subunit
Format:: Supplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 0.05% sodium azide.
Extra Details:: Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.